Mink chromosome N-bands

R-banded Mink chromosomes, restained with N-bands and set up to identify the two pairs of chromosome with N-bands no 2 and 5, where no 5 as can be seen have the stronges band. The N-bands were made as described by Blom & Goodpasture (1976).The N-bands is in according to Mandal et al. (1975) They found a secondary constriction exactly at the same spots where we found the N-bands. Furthermore, they found that there were some variation from cell to cell in the size of the constrictions

The chromosomes with N-bands are connected to the R-banded metaphase with lines, and on the karyotype the site of the N-band are indicated by white lines.

Below seen one more example where chromosome no 5 have the strongest N-band.

The cosmid 34 stains the same regions as the N-banding so it must be considered a clone of rRNA, see picture below. In the upper corner an interphase can be seen showing hybridization to two round structures typical for the nucleolar organizer region. The hybridization signal are fairly strong on the chromosome 5 (the two upper signals). The signal is somewhat weaker on the one of chromosome 2 (both at the bottom, and the weak one is in the middle)

Cosmid 34 has been hybridized to cat and swine chromosomes. It give strong signals in two pig chromosome pairs No 8 and 10 and in cat it give signal only in one pair. Both for pig and cat it is the chromosome known to contain the N-bands.

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